A proteomics sample preparation method for mature, recalcitrant leaves of perennial plants.
Sample preparation is key to the success of proteomics studies.In the present study, two sample preparation methods were tested for their suitability on the mature, recalcitrant leaves of six representative perennial plants (grape, plum, pear, peach, orange, and ramie).An improved sample preparation method was obtained: Tris and Triton X-100 were a